Figure 1a: LB Agar Amp plate of BL21(DE3) E. coli bacteria
with DNA plasmid pGEM-gbr22 and SOC media after 14
hours in a 37°C incubator. Small purple dots are indicative of colonies of bacterial growth.
Figure 1b: Control LB Agar Amp plate of BL21(DE3) E. coli
bacteria with SOC media, without DNA plasmid after 15 hours
in a 37°C incubator.
Figure 1c: LB agar plate without antibiotic of a swab of the
dirt on the floor near lab refrigerator after about 15 hours in
a 37°C incubator; "Fun Plate."
Figure 2: Two 125mL Erlenmeyer flasks of LB and
100ug/ml ampicillin with BL21(DE3) E. coli bacteria
transformed with DNA plasmid pGEM-gbr22 after 24 hours in
a 37°C shaking incubator. Purple/pink color originated
from purple protein produced by bacteria.
Figure 3: Purple cell pellets of BL21(DE3) E. coli bacteria
transformed with DNA plasmid pGEM-gbr22 after 10 minutes
in a 4°C centrifuge at 5,000 rpm. Excess liquid decanted.
Pellet weight recorded as 0.54 g.
Figure 4: PAGE gel of 6 samples of pgbr22. Columns: molecular
weight standard, cell lysate, soluble fraction with pellet, flow through,
wash (20mM imidazole), elution 1, and elution 2
(both elutions done with 1xPBS and 250mM imidazole).
Figure 5: Pre-stained molecular weight standard ladder used in lane 1 of gel sample.
Introduction:
Materials & Methods:
Results:
Figure 1a: LB Agar Amp plate of BL21(DE3) E. coli bacteria
with DNA plasmid pGEM-gbr22 and SOC media after 14
hours in a 37°C incubator. Small purple dots are indicative
of colonies of bacterial growth.
Figure 1b: Control LB Agar Amp plate of BL21(DE3) E. coli
bacteria with SOC media, without DNA plasmid after 15 hours
in a 37°C incubator.
Figure 1c: LB agar plate without antibiotic of a swab of the
dirt on the floor near lab refrigerator after about 15 hours in
a 37°C incubator; "Fun Plate."
Figure 2: Two 125mL Erlenmeyer flasks of LB and
100ug/ml ampicillin with BL21(DE3) E. coli bacteria
transformed with DNA plasmid pGEM-gbr22 after 24 hours in
a 37°C shaking incubator. Purple/pink color originated
from purple protein produced by bacteria.
Figure 3: Purple cell pellets of BL21(DE3) E. coli bacteria
transformed with DNA plasmid pGEM-gbr22 after 10 minutes
in a 4°C centrifuge at 5,000 rpm. Excess liquid decanted.
Pellet weight recorded as 0.54 g.
Figure 4: PAGE gel of 6 samples of pgbr22. Columns: molecular
weight standard, cell lysate, soluble fraction with pellet, flow through,
wash (20mM imidazole), elution 1, and elution 2
(both elutions done with 1xPBS and 250mM imidazole).
Figure 5: Pre-stained molecular weight standard ladder used in lane 1 of gel sample.
Discussion:
Conclusions:
References: